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Gene tagging
 
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Characterizing Sub-Cellular Compartments in Maize Using Fluorescent Protein Tagged Lines

What's new
May 2008 Updates on genetics workshop with Little Big Horn College, MT (May 12-16, 2008) posted here.
April 2008 Fluorescent tag expression images available. Click here to search.
Mar 2008 Seeds available from tagged lines. Click here.
Apr 2006 You can now submit a gene for fluorescent tagging. Go to our FAQ section to find out more information or contact us. The gene submission form is available here.
Jul 2005 Project receives fundings from the National Science Foundation to characterize subcellular localization of maize proteins NSF Award #0501862.
Gene construct statusTotal=54
Tr - Construct tested by transient essay2
C - Construct submitted for maize transformation8
P - Transformed maize plant obtained22
E - Expression of tagged protein verified22
Expression images and moviesTotal=30
Tagging status
Search genes selected for tagging
 
e.g. tubulin, Alpha-zein, RFP
Search confocal images of tag expression
 
e.g. PIN1, YFP, microtubule
Examples of tagged maize genes
Tagged genesPredicted localizationStatus
Tubulin-alphamicrotubulesE
Abphyl1nuclearE
Fea2unknownE
Histone H1BchromatinE
Tonoplast intrinsic protein TIP1vacuolar membraneE
Alpha-zeinprotein bodies, ERE
Ramosa 3unknownE
Pinformed1plasma membrane, vesiclesE
Warty (Rab2A1)vesicles, ER or GolgiE
Last modified: Tuesday, 03-Jun-2008 17:01:34 EDT